Can you find the fake receptor? Coronavirus cannot.


As covid-19 continues to thrive in the U.S., researchers are now developing the next generation of therapeutics, including a new approach that can help reduce the time it takes to recover from the disease.

While there are already treatments antivirals, antibodies, and steroids, scientists in the U.S. and Europe are now focusing on creating decoys of receptors that are commonly linked to the virus, which could potentially neutralize its harmful effects.

To develop the new therapy, scientists first developed mice with a unique human protein known as angiotensin-converting enzyme 2, or ACE2. It lives on the surface of the cells and helps control abnormalities such as inflammation, inflammation, and blood pressure.

While ACE2 receptors are found in cells throughout the body, they are especially prevalent within the lungs, heart, kidneys, and liver-diseased organs. often attacks.

To protect the true ACE2 receptors, here’s how decoy does its job:

Typically, protein spikes on the surface of the virus act like keys to ACE2 receptors, opening the gateway to infection. But decoys, administered intravenously or through the nose depending on the stage of the disease, release the spike protein, carrying it away from the true receptors. After infection, treatment can reduce the viral load inside the body, which may mean the fastest recovery time in patients.

In a study led by Batlle by Daniel, a professor of medicine at Northwestern University, said mice infected with the disease and received treatment had fewer symptoms compared to animals that were not treated, which died.

As of now, only one clinic test of the ACE2 product is completed in patients with moderate to severe symptoms. However, a growing number of researchers are supporting the new therapeutic.

Batlle’s group began working on decoy proteins in January 2020 after learning about the first case in the U.S., advancing knowledge gained from the 2003 SARS-CoV outbreak in China.

“We know it’s likely that the receptor for SARS-CoV-2 could be ACE2, because it has previously been shown to be the case for SARS-CoV,” Batlle said.

But applying that knowledge is not that straightforward. Michael Jewett, a professor of chemical engineering at Northwestern University who was not involved in the study, compared the complicated process of making a decoy to a more brutal puzzle.

“Modifying complex biological systems can be difficult,” Jewett said. “It’s like solving a puzzle and every time you insert a piece, the rest of the puzzle changes.”

Jewett also said that compared to antibody treatments, decoys should be less expensive and easier to use. And some experts are optimistic about the ability of decay to prevent both the original viral strain and mutations from coming.

In another study, using a process called deep mutational scanning, Erik Procko, a professor of biochemistry at the University of Illinois Urbana-Champaign, looked at thousands of different mutations in ACE2 in one experiment and saw which ones are most susceptible to and bind to the virus. His team then built the tricks to emulate the best ever. The decoys do not bind to the cells but float in the fluid between them to capture the virus before it binds to the true ACE2 receptors.

By using a combination of three mutations, his team was able to increase the decoy correlation for covid-19. They produce decoy receptors that bind to the virus 50 times more strongly than ACE2.

To test the method, Procko’s team used mouse tissue instead of live animals. “In in vitro tissue culture, we find that some of the decoy receptors are equally potent-sometimes quite fine, sometimes slightly smaller, but generally as potent-as monoclonal antibodies with permission to be used. in an emergency or in clinical trials, ”Procko said.

One concern is that one of its mutations could allow so-called viral escape and help improve viral resistance to treatment. But because decoys are almost identical to natural receptors, according to Procko, the virus may not develop unnaturally because of their action.

Due to differences in infrastructure and education, access to synthetic-biology technologies is not equally provided around the world. A lot of research – and a lot of funding – is needed before the public can use such therapy. But advances like these could help create low-cost, portable, easy-to-use treatments for the disease.

“There are reassuring signs that decoys that are very close to the human ACE2 receptor could be potent and effective against all of these new varieties,” Procko said. “I wouldn’t be surprised if we had some of the next generation come to the clinic in two years.”



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